AbD Serotec/Trastuzumab antibody | AbD18018/0.1 mg/HCA168,AbD Serotec,,AbD Serotec,AbDSerotec,bio-rad-antibodies,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：AbD Serotec/Trastuzumab antibody | AbD18018/0.1 mg/HCA168

产品编号：HCA168

市场价：¥0.00

场地：美国(厂家直采)

产品分类：分子类>克隆和表达>基因突变>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： AbD Serotec

公司：AbDSerotec

公司分类：

商品介绍

Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents

Human Anti-Trastuzumab Antibody, clone AbD18018, is a paratope specific, high affinity, anti-idiotypic antibody that recognizes the monoclonal antibody drug trastuzumab and inhibits it binding to its target. The antibody can be used to measure the levels of trastuzumab and biosimilar products in bioanalytical assays.Human Anti-Trastuzumab Antibody, clone AbD18018 is a fully human recombinant monoclonal antibody in monovalent Fab format and is suitable as a capture or detection antibody paired with antibody clone AbD16712 (HCA166) in a pharmacokinetic (PK) bridging ELISA, to measure free drug in preclinical research and clinical trials using patient sera.Human Anti-Trastuzumab Antibody, clone AbD18018 is also available in full immunoglobulin format as IgG1 isotype (HCA177) or IgG4 isotype (HCA270) in order to offer assay developers increased flexibility in assay design.Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.View a summary of all Anti-Trastuzumab Antibodies.

Product Details

Product Form: A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation: Metal chelate affinity chromatography
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.01% Thiomersal
Immunogen: Trastuzumab
Affinity: HCA168 is an ultra-high affinity idiotype antibody that specifically recognises the Herceptin humanised monoclonal antibody. The monovalent intrinsic affinity of HCA168 was measured as 0.02nM by real time, label-free molecular interaction analysis using an Attana A200 instrument on immobilized Herceptin.
Approx. Protein Concentrations: Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.Herceptin is a registered trademark of Genentech, Inc.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro research testing services. Any re-sale or any other form of commercial application needs a written agreement with Bio-Rad.
Regulatory: For research purposes only

Applications of Trastuzumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in theHuCAL Antibodies Technical Manual
ELISA: This product may be used in a direct ELISA or as a capture reagent in a sandwich ELISA together with HCA166 as the detection reagent, when conjugated to HRP.Protocol: PK bridging ELISA to measure free drug

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Rabbit anti DYKDDDDK Tag:HRP	AHP1074P	E WB	0.1 mg	Log in	Get Quote
Goat anti Histidine Tag:Biotin	AHP1656B	E WB	0.1 mg	Log in	Get Quote
Mouse anti Histidine Tag:Alk.Phos.	MCA1396A	E WB	0.1 mg	Log in	Get Quote
Mouse anti Histidine Tag:Biotin	MCA1396B	E WB	0.1 mg	Log in	Get Quote
Rat anti DYKDDDDK Tag:HRP	MCA4764P	E P WB	0.1 mg	Log in	Get Quote
Mouse anti Penta Histidine Tag:HRP	MCA5995P	E WB	125 µl	Log in	Get Quote

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
HuCAL Fab-FH Negative Control	HCA045	E	0.1 mg	Log in		Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Hispec Assay Diluent	BUF049A	E IY	50 ml	Log in	Get Quote
Human anti Trastuzumab	HCA166	E	0.1 mg	Log in	Get Quote
Human anti Trastuzumab	HCA167	E	0.1 mg	Log in	Get Quote
Human anti Trastuzumab	HCA169	E	0.1 mg	Log in	Get Quote
Human anti Trastuzumab	HCA176	E	0.1 mg	Log in	Get Quote
Human anti Trastuzumab	HCA177	E	0.1 mg	Log in	Get Quote
Mouse anti Human IgG (Fc) CH2 Domain:HRP	MCA647P	C* E	0.2 mg	Log in	Get Quote