AbD Serotec/Golimumab antibody | AbD25418_hIgG1/0.1 mg/HCA287P,AbD Serotec,,AbD Serotec,AbDSerotec,bio-rad-antibodies,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：AbD Serotec/Golimumab antibody | AbD25418_hIgG1/0.1 mg/HCA287P

产品编号：HCA287P

市场价：¥0.00

场地：美国(厂家直采)

产品分类：分子类>克隆和表达>克隆试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： AbD Serotec

公司：AbDSerotec

公司分类：

商品介绍

Summary
Useful Reagents

Human Anti-Golimumab Antibody, clone AbD25418_hIgG1 is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the free human monoclonal antibody golimumab and inhibits binding to its target TNFα.Clone AbD25418_hIgG1 does not recognize free human TNFα or golimumab in complex with human TNFα. This antibody can be used to measure free golimumab and biosimilar products in bioanalytical assays.Clone AbD25418_hIgG1 is an affinity-matured variant of AbD20692 (HCA241), with about a 21-fold affinity improvement. A pair of anti golimumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody paired with clone AbD25429 (HCA286) in monovalent Fab format as the capture antibody.Golimumab, marketed under the brand name Simponi is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.View a summary of all anti-golimumab antibodies

Product Details

Product Form: Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) – liquid
Product Form: Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Thiomersal
Preservative Stabilisers: 0.01% Thiomersal
Immunogen: Golimumab
Affinity: The monovalent intrinsic affinity of AbD25418 was measured as K_D = 0.13 nM by real time, label free molecular interaction analysis on immobilized golimumab
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml

Storage Information

Storage: Store at -70^oC.Storage in frost-free freezers is not recommended.This product should be stored undiluted.
Storage: Store at +4^oC or at -20^oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch

More Information

Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe
Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe
Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad
Regulatory: For research purposes only

Applications of Golimumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in theHuCAL Antibodies Technical Manual
Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in theHuCAL Antibodies Technical Manual
ELISA: This product may be used in a direct ELISA or as detection antibody for golimumab in a bridging ELISA together with HCA286 (AbD25429, Fab-FH) as the capture reagent.Protocol: PK bridging ELISA to measure free drug
ELISA: This product may be used in a direct or indirect ELISA or as detection antibody for golimumab in a bridging ELISA together with HCA286 (AbD25429) as the capture reagent.Protocol: PK bridging ELISA to measure free drug

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Hispec Assay Diluent	BUF049A	E IY	50 ml	Log in	Get Quote
Human anti Golimumab	HCA286	E	0.1 mg	Log in	Get Quote
Hispec Assay Diluent	BUF049A	E IY	50 ml	Log in	Get Quote
Human anti Golimumab	HCA286	E	0.1 mg	Log in	Get Quote
Human anti Golimumab:HRP	HCA287P	E	0.1 mg	Log in	Get Quote
LYNX Rapid HRP Antibody Conjugation Kit	LNK001P	CJ	1 Conjugation For 400µg Antibody	Log in	Get Quote
Mouse anti Human IgG (Fc) CH2 Domain:HRP	MCA647P	C* E	0.2 mg	Log in	Get Quote