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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
AbD Serotec/IFN Gamma antibody | 7B6/0.5 mg/MCA1964
产品编号:MCA1964
市  场 价:¥0.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: AbD Serotec
公      司:AbDSerotec
公司分类:
AbD Serotec/IFN Gamma antibody | 7B6/0.5 mg/MCA1964
商品介绍
Mouse anti Bovine interferon-γ antibody, clone 7B6 recognises both native and recombinant bovine IFN-γ. Bovine interferon-γ is a 166 amino acid ~18 kDa secreted cytokine with antiviral activity. Interferon-γ, secreted by lymphocytes in response to specific mitogens, is also a potent macrophage activator and has antiproliferative actions on transformed cells and can enhance functions of type-1 interferons.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Fallow deer
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P07353
Entrez Gene
IFNG
GO Terms
GO:0009615response to virus
GO:0005125cytokine activity
GO:0005133interferon-gamma receptor binding
GO:0005615extracellular space
GO:0006955immune response
Regulatory
For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application NameVerifiedMin DilutionMax Dilution
ELISA
Flow Cytometry 11/5001/2000
Immunohistology - Paraffin 2
Western Blotting
  1. 1Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09A) is recommended for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

DescriptionProduct CodeApplicationsPack SizeList PriceQuantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117AE WB 0.5 mgloader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GAF IF 0.1 mgloader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GAF WB 0.1 mgloader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GAF IF WB 0.1 mgloader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117FF 0.5 mgloader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117PE WB 0.5 mgloader
Goat anti Mouse IgG (Fc):FITCSTAR120FC F 1 mgloader
Goat anti Mouse IgG (Fc):HRPSTAR120PE WB 1 mgloader
Rabbit F(ab')2 anti Mouse IgG:RPESTAR12AF 1 mlloader
Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR70F 0.5 mgloader
Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR76F 1 mlloader
Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR77C E P 0.5 mgloader
Goat anti Mouse IgG/A/M:Alk. Phos.STAR87AC E WB 1 mgloader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87PE 1 mgloader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GAF IF WB 0.1 mgloader
Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9BF 1 mgloader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13BC E P RE WB 1 mgloader

Negative Isotype Controls Available

DescriptionProduct CodeApplicationsPack SizeList PriceQuantity
Mouse IgG1 Negative ControlMCA928F 100 Testsloader

Application Based External Images

Flow Cytometry

Immunohistology - Paraffin

Product Specific References

References for IFN Gamma antibody

  1. Weynants, V. et al. (1998) Quantitative assessment by flow cytometry of T-lymphocytes producing antigen-specific gamma-interferon in Brucella immune cattle.Vet Immunol Immunopathol. 66 (3-4): 309-20.
  2. Esteves, I. et al. (2004) Protective killed Ehrlichia ruminantium vaccine elicits IFN-gamma responses by CD4+ and CD8+ T lymphocytes in goats.Vet Immunol Immunopathol. 98 (1-2): 49-57.
  3. Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.Clin Vaccine Immunol. 17: 108-17.
  4. Stevens, E.T. et al. (2009) The induction of a cell-mediated immune response to bovine viral diarrhea virus with an adjuvanted inactivated vaccine.Vet Ther. 10: E1-8.
  5. Lei, L. et al. (2008) Live Mycobacterium avium subsp. paratuberculosis and a killed-bacterium vaccine induce distinct subcutaneous granulomas, with unique cellular and cytokine profiles.Clin Vaccine Immunol. 15: 783-93.
  6. Rogers, A.N. et al. (2005) Gammadelta T cell function varies with the expressed WC1 coreceptor.J Immunol. 174: 3386-93.
  7. Rosbottom, A. et al. (2008) Upregulation of cytokines is detected in the placentas of cattle infected with Neospora caninum and is more marked early in gestation when fetal death is observed.Infect Immun. 76 (6): 2352-61.
  8. García-Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama).Vet Immunol Immunopathol. 149: 66-75.
  9. Canal, A.M. et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.Res Vet Sci. 110: 34-39.
  10. Stevens, E.T. et al. (2009) The induction of a cell-mediated immune response to bovine viral diarrhea virus with an adjuvanted inactivated vaccine.Vet Ther. 10 (4): E1-8.
  11. Çomakli, S. & Özdemir, S. (2019) Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1.Pathogens. 8 (1)Feb 25 [Epub ahead of print].

Fluorescent Spectraviewer

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How to Use the Spectraviewer?

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra
View more products with IFN GAMMA specificity
品牌介绍

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