商品介绍
Mouse anti Bovine interferon-γ antibody, clone 7B6 recognises both native and recombinant bovine IFN-γ. Bovine interferon-γ is a 166 amino acid ~18 kDa secreted cytokine with antiviral activity. Interferon-γ, secreted by lymphocytes in response to specific mitogens, is also a potent macrophage activator and has antiproliferative actions on transformed cells and can enhance functions of type-1 interferons.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
Target Species Cross Reactivity Sheep Fallow deer Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P07353
- Entrez Gene
- IFNG
- GO Terms
- GO:0009615response to virus
- GO:0005125cytokine activity
- GO:0005133interferon-gamma receptor binding
- GO:0005615extracellular space
- GO:0006955immune response
- Regulatory
- For research purposes only
Applications of IFN Gamma antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry 1 | 1/500 | 1/2000 | |
| Immunohistology - Paraffin 2 | |||
| Western Blotting |
- 1Membrane permeabilization is required for this application. The use of Leucoperm™ (Product Code BUF09A) is recommended for this purpose.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E WB | 0.5 mg |  | |
| Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) | STAR117D488GA | F IF | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) | STAR117D680GA | F WB | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | F IF WB | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg | | |
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | E WB | 0.5 mg | | |
| Goat anti Mouse IgG (Fc):FITC | STAR120F | C F | 1 mg | | |
| Goat anti Mouse IgG (Fc):HRP | STAR120P | E WB | 1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml | | |
| Goat anti Mouse IgG:FITC (Rat Adsorbed) | STAR70 | F | 0.5 mg | | |
| Goat anti Mouse IgG:RPE (Rat Adsorbed) | STAR76 | F | 1 ml | | |
| Goat anti Mouse IgG:HRP (Rat Adsorbed) | STAR77 | C E P | 0.5 mg | | |
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg | | |
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:Dylight®800 | STAR8D800GA | F IF WB | 0.1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg | |
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests | |
Application Based External Images
Flow Cytometry
Immunohistology - Paraffin
Product Specific References
References for IFN Gamma antibody
- Weynants, V. et al. (1998) Quantitative assessment by flow cytometry of T-lymphocytes producing antigen-specific gamma-interferon in Brucella immune cattle.Vet Immunol Immunopathol. 66 (3-4): 309-20.
- Esteves, I. et al. (2004) Protective killed Ehrlichia ruminantium vaccine elicits IFN-gamma responses by CD4+ and CD8+ T lymphocytes in goats.Vet Immunol Immunopathol. 98 (1-2): 49-57.
- Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.Clin Vaccine Immunol. 17: 108-17.
- Stevens, E.T. et al. (2009) The induction of a cell-mediated immune response to bovine viral diarrhea virus with an adjuvanted inactivated vaccine.Vet Ther. 10: E1-8.
- Lei, L. et al. (2008) Live Mycobacterium avium subsp. paratuberculosis and a killed-bacterium vaccine induce distinct subcutaneous granulomas, with unique cellular and cytokine profiles.Clin Vaccine Immunol. 15: 783-93.
- Rogers, A.N. et al. (2005) Gammadelta T cell function varies with the expressed WC1 coreceptor.J Immunol. 174: 3386-93.
- Rosbottom, A. et al. (2008) Upregulation of cytokines is detected in the placentas of cattle infected with Neospora caninum and is more marked early in gestation when fetal death is observed.Infect Immun. 76 (6): 2352-61.
- García-Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama).Vet Immunol Immunopathol. 149: 66-75.
- Canal, A.M. et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.Res Vet Sci. 110: 34-39.
- Stevens, E.T. et al. (2009) The induction of a cell-mediated immune response to bovine viral diarrhea virus with an adjuvanted inactivated vaccine.Vet Ther. 10 (4): E1-8.
- Çomakli, S. & Özdemir, S. (2019) Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1.Pathogens. 8 (1)Feb 25 [Epub ahead of print].
Fluorescent Spectraviewer
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- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
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- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
View more products with IFN GAMMA specificity
品牌介绍
AbD Serotec为了帮助您实现研究目标，Bio-Rad提供了广泛的测定试剂盒组合，用于研究各种生物过程和研究领域，例如癌症，关节炎或阿尔茨海默氏病。该套件设计为易于使用并提供一致的结果。
为了分析细胞凋亡，我们提供了基于凋亡过程不同方面的各种细胞凋亡检测试剂盒。我们的Magic Red™组织蛋白酶试剂盒使用一种快速简便的方法来分析细胞内组织蛋白酶，该过程涉及自噬和细胞凋亡。与基于常规抗体或ELISA的检测方法相反，该方法适用于荧光显微镜或荧光板读取器。
我们还提供了完整的随时可用的抗体纯化试剂盒，这些试剂盒旨在消除通常与蛋白A色谱法相关的繁琐色谱步骤。
对于内毒素的去除，我们的纯化试剂盒可快速，一步和高质量地从重组蛋白，抗体和病毒载体中去除内毒素。我们还提供用于ELISA，大鼠单克隆抗体同型分型和IHC实验的试剂盒，以帮助您更快地获得准确一致的结果。
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