Rabbit anti Bovine interleukin-17A antibody recognizes bovine interleukin-17A (IL-17A), a 35 kDa disulfide-linked homodimer consisting of two 153 amino acid chains, which belongs to the IL-17 family (classified as IL-17A to IL-17F), and signals through the IL-17 receptor (IL-17R/CDw217).IL-17, originally thought to have been produced primarily by activated CD4+ T cells, appears to have a range of cellular origins and induces a variety of target cells to secrete inflammatory cytokines, including IL-1β, IL-6, TNFα, IFNγ and granulocyte colony-stimulating factor, as well as synergizing with other inflammatory cytokines and agonists.
- Target Species
- Bovine
- Species Cross-Reactivity
Target Species | Cross Reactivity |
---|
Dolphin | |
Dog | Weak |
Horse | Weak |
Mouse | |
Sheep | |
Pig | |
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to bovine interleukin-17A were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- Recombinant bovine interleukin-17A
- Approx. Protein Concentrations
- Total protein concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Storage
- Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- Storage
- Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- UniProt
- Q687Y7
- Entrez Gene
- IL17A
- GO Terms
- GO:0005125cytokine activity
- GO:0005615extracellular space
- GO:0006954inflammatory response
- Regulatory
- For research purposes only
Applications of IL-17A antibody
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.Application Name | Verified | Min Dilution | Max Dilution |
---|
ELISA | | 0.01 ug/ml | 0.5 ug/ml |
Western Blotting | | 0.1 ug/ml | 1.0 ug/ml |
ELISA | | 1.0 ug/ml | 10 ug/ml |
Western Blotting | | 0.1 ug/ml | 1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used as a detection reagent in a sandwich ELISA together with AHP2376 as the capture reagent. Recombinant bovine interleukin-17A (PBP025) may be used as a standard.
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with AHP2376B as the detection reagent. Recombinant bovine interleukin-17A (PBP025) may be used as a standard.
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Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | Your Price | List Price | Quantity |
---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg | Log in |  | |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg | Log in |  | |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg | Log in |  | |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg | Log in |  | |
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg | Log in |  | |
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml | Log in |  | |
Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | Log in | | |
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | Log in | | |
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg | Log in | | |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | Your Price | List Price | Quantity |
---|
Rabbit anti Bovine Interleukin-17A | AHP2376 | E WB | 0.1 mg | Log in |  | |
Recombinant Bovine Interleukin-17A | PBP025 | E | 5 µg | Log in |  | |
Rabbit anti Bovine Interleukin-17A:Biotin | AHP2376B | E WB | 50 µg | Log in |  | |
Recombinant Bovine Interleukin-17A | PBP025 | E | 5 µg | Log in |  | |
Fluorescent Spectraviewer
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How to Use the Spectraviewer?
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- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra