商品介绍
Human anti Trastuzumab antibody, clone AbD16712 a monoclonal antibody recognizes Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Herceptin binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.Human anti Trastuzumab (AbD16712) is an anti-idiotypic antibody that specifically recognizes the Herceptin humanised monoclonal antibody and can be used to measure the Heceptin levels in serum from patients.This clone has been demonstrated to inhibit the Herceptin - HER2 interaction. Removal of the preservative is recommended prior to use in functional assays - Bio-Rad recommend the use of a Slide-A-Lyser® dialysis cassette (EQU003) for this purpose.Additional Trastuzumab (Herceptin) specific anti-idiotypic antibodies are available upon request
Product Details
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. Supplied as a liquid.
- Preparation
- StrepTactin affinity chromatography
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Herceptin
- Affinity
- The monovalent intrinsic affinity of HCA166 was measured as 0.4nM by real time, label-free molecular interaction analysis using an Attana A200 instrument on immobilized Herceptin.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents.This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.Herceptin® is a registered trademark of Genentech, Inc.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Trastuzumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in theHuCAL Antibodies Technical Manual
- ELISA
- This product may be used as a detection antibody, or as capture antibody in a sandwich ELISA together with HCA168, when conjugated to HRP, as the detection reagent.Protocol: PK bridging ELISA to measure free drug
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti V5-Tag:Alk. Phos. | MCA1360A | E WB | 0.1 mg |  | |
| Mouse anti V5-Tag:Biotin | MCA1360B | C E WB | 0.1 mg | | |
| Mouse anti V5-Tag:HRP | MCA1360P | E WB | 0.1 mg | | |
| Mouse anti Strep-Tag Immo | MCA2488 | E | 0.1 mg | | |
| Mouse anti Strep-Tag Classic:HRP | MCA2489P | E WB | 75 µl | |
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| HuCAL Fab-V5Sx2 Negative Control | HCA125 | E | 0.1 mg | |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Trastuzumab | HCA167 | E | 0.1 mg | | |
| Human anti Trastuzumab | HCA168 | E | 0.1 mg | | |
| Human anti Trastuzumab | HCA169 | E | 0.1 mg | | |
| Human anti Trastuzumab | HCA176 | E | 0.1 mg | | |
| Human anti Trastuzumab | HCA177 | E | 0.1 mg | | |
| Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C* E | 0.2 mg | |
View more products with Trastuzumab specificity
品牌介绍
AbD Serotec为了帮助您实现研究目标，Bio-Rad提供了广泛的测定试剂盒组合，用于研究各种生物过程和研究领域，例如癌症，关节炎或阿尔茨海默氏病。该套件设计为易于使用并提供一致的结果。
为了分析细胞凋亡，我们提供了基于凋亡过程不同方面的各种细胞凋亡检测试剂盒。我们的Magic Red™组织蛋白酶试剂盒使用一种快速简便的方法来分析细胞内组织蛋白酶，该过程涉及自噬和细胞凋亡。与基于常规抗体或ELISA的检测方法相反，该方法适用于荧光显微镜或荧光板读取器。
我们还提供了完整的随时可用的抗体纯化试剂盒，这些试剂盒旨在消除通常与蛋白A色谱法相关的繁琐色谱步骤。
对于内毒素的去除，我们的纯化试剂盒可快速，一步和高质量地从重组蛋白，抗体和病毒载体中去除内毒素。我们还提供用于ELISA，大鼠单克隆抗体同型分型和IHC实验的试剂盒，以帮助您更快地获得准确一致的结果。
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