商品介绍
Mouse anti Bovine TNF alpha antibody, clone CC327 recognizes bovine TNF alpha, a 17.5 kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes.The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
Target Species Cross Reactivity Fallow deer - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant bovine TNF alpha.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Storage
- Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q06599
- Entrez Gene
- TNF
- GO Terms
- GO:0005125cytokine activity
- GO:0005615extracellular space
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of TNF Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | 1/10 | 1/50 | |
| ELISA | |||
| Flow Cytometry 1 |
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- ELISA
- This reagent may be used as a capture antibody in a sandwich ELISA for bovine TNF alpha in combination with MCA2335B as detection reagent. PBP005 may be used as a standard.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E WB | 0.5 mg |  | |
| Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) | STAR117D488GA | F IF | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) | STAR117D680GA | F WB | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | F IF WB | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg | | |
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | E WB | 0.5 mg | | |
| Goat anti Mouse IgG (Fc):FITC | STAR120F | C F | 1 mg | | |
| Goat anti Mouse IgG (Fc):HRP | STAR120P | E WB | 1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml | | |
| Goat anti Mouse IgG:FITC (Rat Adsorbed) | STAR70 | F | 0.5 mg | | |
| Goat anti Mouse IgG:RPE (Rat Adsorbed) | STAR76 | F | 1 ml | | |
| Goat anti Mouse IgG:HRP (Rat Adsorbed) | STAR77 | C E P | 0.5 mg | | |
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg | | |
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:Dylight®800 | STAR8D800GA | F IF WB | 0.1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg | |
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2b Negative Control:Alexa Fluor® 488 | MCA691A488 | F | 100 Tests/1ml | | |
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests | |
Application Based External Images
ELISA
Product Specific References
References for TNF Alpha antibody
- Hope, J.C. et al. (2003) Maturation of bovine dendritic cells by lipopeptides.Vet Immunol Immunopathol. 95 (1-2): 21-31.
- Whelan, A.O. et al. (2003) Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide. Infect Immun. 71 (11): 6420-5.
- Guergnon J et al. (2003) A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaβ activation of Theileria parva-transformed B cells.Cell Microbiol. 5 (10): 709-16.
- Kwong, L.S. et al. (2010) Production and characterization of two monoclonal antibodies to bovine tumournecrosis factor alpha (TNF-alpha) and their cross-reactivity with ovine TNF-alpha.Vet Immunol Immunopathol. 135: 320-4.
- Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.J Dairy Sci. 93: 2458-70.
- Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.Funct Integr Genomics. 10: 21-38.
- Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24.
- Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.Vet Res. 42: 49.
- Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.PLoS One. 6: e29194.
- García-Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama).Vet Immunol Immunopathol. 149: 66-75.
- Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.J Comp Pathol. 150 (4): 434-48.
- Luisa, C. et al. (2016) Evaluation of serum markers of blood redox homeostasis and inflammation in PCB naturally contaminated heifers undergoing decontaminationScience of The Total Environment. 542: 653-64.
- Maggioli, M.F. et al. (2016) Increased TNF-α/IFN-γ/IL-2 and Decreased TNF-α/IFN-γ Production by Central Memory T Cells Are Associated with Protective Responses against Bovine Tuberculosis Following BCG Vaccination.Front Immunol. 7: 421.
- Rutigliano, H.M. et al. (2016) Trophoblast Major Histocompatibility Complex Class I Expression Is Associated with Immune-Mediated Rejection of Bovine Fetuses Produced by Cloning.Biol Reprod. 95 (2): 39.
- Camejo, M.I. et al. (2014) TNF-alpha in bulls experimentally infected with Trypanosoma vivax: a pilot study.Vet Immunol Immunopathol. 162 (3-4): 192-7.
- Jolly A et al. (2016) Evidence of a pro-apoptotic effect of specific antibodies in a bovine macrophage model of infection with Mycobacterium avium subsp. paratuberculosis.Vet Immunol Immunopathol. 169: 47-53.
Fluorescent Spectraviewer
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Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
View more products with TNF ALPHA specificity
品牌介绍
AbD Serotec为了帮助您实现研究目标，Bio-Rad提供了广泛的测定试剂盒组合，用于研究各种生物过程和研究领域，例如癌症，关节炎或阿尔茨海默氏病。该套件设计为易于使用并提供一致的结果。
为了分析细胞凋亡，我们提供了基于凋亡过程不同方面的各种细胞凋亡检测试剂盒。我们的Magic Red™组织蛋白酶试剂盒使用一种快速简便的方法来分析细胞内组织蛋白酶，该过程涉及自噬和细胞凋亡。与基于常规抗体或ELISA的检测方法相反，该方法适用于荧光显微镜或荧光板读取器。
我们还提供了完整的随时可用的抗体纯化试剂盒，这些试剂盒旨在消除通常与蛋白A色谱法相关的繁琐色谱步骤。
对于内毒素的去除，我们的纯化试剂盒可快速，一步和高质量地从重组蛋白，抗体和病毒载体中去除内毒素。我们还提供用于ELISA，大鼠单克隆抗体同型分型和IHC实验的试剂盒，以帮助您更快地获得准确一致的结果。
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