Chicken anti Bovine interleukin-6 antibody recognizes bovine interleukin-6 (IL-6), a 23.8 kDa cytokine belonging to the IL-6 superfamily.Bovine interleukin-6 is a multifunctional cytokine secreted by a range of cell types, in particular T lymphocytes and macrophages. IL-6 has a range of activities and includes the differentiation of B cells into Ig producing cells, differentiation of lymphocytes and of monocytes. IL-6 functions in both a pro-inflammatory role where it is an important inducer of the acute phase response and also as an anti-inflammatory myokine (Febbraio & Pedersen 2005).
- Target Species
- Bovine
- Species Cross-Reactivity
Target Species | Cross Reactivity |
---|
Sheep | Weak |
Cat | |
Dog | |
Horse | |
Human | |
Mouse | |
Pig | |
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to bovine interleukin-6 were raised by repeated immunisation of chickens with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant bovine interleukin-6
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Storage
- Store at +4oC. DO NOT FREEZE.This product should be stored undiluted.
- Storage
- Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- UniProt
- P26892
- Entrez Gene
- IL6
- GO Terms
- GO:0006953acute-phase response
- GO:0005125cytokine activity
- GO:0005615extracellular space
- GO:0006955immune response
- GO:0008083growth factor activity
- Regulatory
- For research purposes only
Applications of IL-6 antibody
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.Application Name | Verified | Min Dilution | Max Dilution |
---|
ELISA | | 0.1ug/ml | 0.5ug/ml |
ELISA | | 1ug/ml | 5ug/ml |
Western Blotting | | 0.1ug/ml | 2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used as a detection antibody in a sandwich ELISA together with AHP2380 as the capture reagent. Recombinant Bovine interleukin-6 (PBP021) may be used as a standard.
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with AHP2380B as the detection reagent. Recombinant Bovine interleukin-6 (PBP021) may be used as a standard.
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